Application

Immunoblotting: 1:3,000 using TBS (not PBS) and blocking is best performed with IgG free BSA highest quality. Antibody recognizes ~75 kDa band in non-acid-treated serum samples corresponding to the GDNF/GDNFR complex. When samples are acid treated, a prominent 32 kDa (dimer) and weaker 16 kDa monomer are observed. Acid treatment: homogenized tissue samples in PBS are acid-treated by adding 1N HCL until the pH is less than 3.0 (pH paper strips), incubated 15 minutes at room temperature and then neutralized by adding 1N NaOH to pH 7.6.

Optimal working dilutions must be determined by end user.

Anti-Glial Derived Neurotrophic Factor Antibody detects level of Glial Derived Neurotrophic Factor & has been published & validated for use in WB.

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & NeurotrophinsNeuroinflammation & Pain

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant human GDNF.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Sheep serum. Lyophilized. Reconstitute with 100 µL of sterile distilled water. Centrifuge to remove any residue. Contains no preservative. Glycerol (1:1) can be added for additional stability.

Specificity

Specific for GDNF. No cross reactivity with Neurturin.

Storage and Stability

Maintain lyophilized material at -20°C to -70°C (dry) for up to twelve months. After reconstitution maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.